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objective signs may include tachycardia, hypotension, change in mental status, severe cardiovascular collapse, unconsciousness, and death

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For subculturing, the cells were detached from the surface of the flask with a 0.1% trypsin/EDTA solution (Gibco, USA), transferred to 50-mL Falcon tubes, centrifuged (8 min, 1,600 rpm, 4C), and resuspended in a fresh culture medium

Additional, more stringent wash buffers are used to remove hydrophobically bound and precipitated proteins

contortus , we further analyzed the differences in the proteomes of the two strains (ISE and IRE) using isobaric labeling with tandem mass tags (TMT) followed by high-resolution LCMS/MS